{"product_id":"abcam-ab263175","title":"Abcam, ab263175, Human DPM2 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nDPM2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon2 and 4 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon2 and 4 bp deletion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-DPM2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDPM2 also known as dolichyl-phosphate mannosyltransferase polypeptide 2 is a component of the dolichol-phosphate mannose (DPM) synthase complex. This protein weighs approximately 25 kDa and plays a significant role in mannose transfer. It is ubiquitously expressed in various tissues with higher expression levels in the liver heart and muscle. The DPM2 works as a regulator and stabilizer within the DPM synthase complex essential for glycosylation processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDPM2 is involved in the assembly of the glycosylation precursor dolichol-phosphate-mannose which is critical for the proper synthesis of glycoproteins. As part of the DPM synthase complex DPM2 teams with the catalytic subunit DPM1 and the anchoring subunit DPM3. These interactions ensure effective glycosylation necessary for protein folding and function. The glycosylation process is essential for the stability and activity of numerous proteins within eukaryotic organisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nDPM2 plays a central role in the protein glycosylation pathway and the synthesis of glycosylphosphatidylinositol (GPI) anchors. It works closely with DPM1 activating it to transfer mannose molecules from GDP-mannose to dolichol-phosphate an important step in the formation of GPI-anchored proteins. These pathways are vital for cell surface expression and protein localization implicating DPM2 in broader cell signaling and communication networks.\u003cbr\u003e\nDPM2 mutations are linked to Congenital Disorders of Glycosylation (CDG) particularly CDG-Iu. These conditions affect multiple systems due to improper glycosylation leading to symptoms ranging from developmental delays to organ dysfunction. Furthermore DPM2 in association with DPM1 and other partners in the DPM synthase complex has connections to muscular dystrophies due to its role in muscle glycosylation processes. Understanding these relationships is important for developing therapeutic interventions targeting glycosylation-related disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845175824553,"sku":"ab263175","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263175","provider":"Iright","version":"1.0","type":"link"}