{"product_id":"abcam-ab263207","title":"Abcam, ab263207, Human FXYD5 (Dysadherin) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nFXYD5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-FXYD5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDysadherin also known as FXYD domain-containing ion transport regulator 5 (FXYD5) is a small protein with a mass of approximately 20 kDa. It localizes on the cell surface as a glycoprotein. The protein has wide expression across various tissues including epithelial tissues and certain cancerous cells. Dysadherin has been primarily identified in cells involved in malignancy and metastasis which suggests its role in cancer biology.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDysadherin contributes to cell-cell adhesion disruption facilitating the metastatic spread of cancer cells. It is not part of a known protein complex but it interacts with the extracellular matrix to alter cellular cohesion and mobility. By influencing the cellular adhesion properties dysadherin may play a part in the invasive characteristics of some epithelial tumors. This alteration can drive processes leading to carcinoma progression by reducing cell-cell interaction stability.\u003cbr\u003e\nPathways\u003cbr\u003e\nDisruptions in cell adhesion mediated by proteins like dysadherin can impact the epithelial-mesenchymal transition (EMT) pathway significantly. Within this pathway dysadherin decreases E-cadherin expression which weakens cellular adhesion enhancing cell motility and invasiveness. Also it may engage in the PI3K\/AKT signaling pathway by affecting cellular growth and survival. Dysadherin might act together with other regulatory proteins that modulate cell adhesion and mobility impacting tumor behavior and progression.\u003cbr\u003e\nDysadherin's expression links closely with metastatic cancers including colorectal cancer and breast cancer. Its role in reducing E-cadherin function means it connects to diseases where diminished cell adhesion contributes to cancer metastasis. Dysadherin has associations with other proteins implicated in these diseases such as integrins which also influence metastatic cell movement and invasion. Understanding dysadherin's function and interactions may provide insight into therapeutic strategies for tackling metastatic disease.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855782367401,"sku":"ab263207","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263207","provider":"Iright","version":"1.0","type":"link"}