{"product_id":"abcam-ab263236","title":"Abcam, ab263236, Human ITFG1 (CDA08) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nITFG1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Insertion of the selection cassette in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Insertion of the selection cassette in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ITFG1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCDA08 also known as cytidine deaminase weighs approximately 16 kDa and is expressed mainly in the spleen liver and bone marrow. This enzyme catalyzes the deamination of cytidine transforming cytidine into uridine and plays a role in nucleotide metabolism. By conducting this reaction CDA08 regulates cytidine and deoxycytidine levels impacting DNA and RNA synthesis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme CDA08 modulates cell proliferation and apoptosis through its regulation of nucleotide pools. It is not part of any known larger protein complex standing as a singular enzyme in its function. CDA08 activity influences the balance between pyrimidine metabolites impacting cellular functions such as DNA repair and replication.\u003cbr\u003e\nPathways\u003cbr\u003e\nScientists have linked CDA08 to the pyrimidine metabolism and nucleic acid synthesis pathways. Its activity integrates with key enzymes in these processes maintaining cellular nucleotide homeostasis. Additionally proteins like ribonucleotide reductase interact functionally with CDA08 showing a coordinated regulation of deoxycytidine triphosphate levels essential for DNA synthesis.\u003cbr\u003e\nResearchers have connected CDA08 with acute myeloid leukemia and certain lymphomas. Alterations in CDA08 expression or activity can affect chemotherapeutic drug metabolism influencing treatment outcomes. Furthermore proteins such as deoxycytidine kinase are related to CDA08 through their roles in these disorders linking drug resistance mechanisms with nucleotide metabolism.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845190799529,"sku":"ab263236","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263236","provider":"Iright","version":"1.0","type":"link"}