{"product_id":"abcam-ab263237","title":"Abcam, ab263237, Human IWS1 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nIWS1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Insertion of the selection cassette in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Insertion of the selection cassette in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-IWS1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Integrator Complex Subunit 1 (IWS1) is a protein that plays an important role in RNA processing and transcriptional regulation. It is also known by other names such as Factor That Associates with Transcription Elongation Complex 6 (FACT Complex) and Suppressor of Ty6 Homolog (Spt16). IWS1 has a molecular mass of approximately 105 kDa. The expression of IWS1 is widespread with high levels observed in actively dividing cells especially in proliferative tissues and cancer cells indicating its important role in cell growth and division processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe IWS1 protein is involved in mediating interactions between the transcription machinery and chromatin. IWS1 often functions as a part of the transcription elongation complex enhancing the efficiency of RNA polymerase II during the elongation phase of transcription. IWS1 as part of this complex assists in chromatin remodeling which allows for the transcription of tightly packaged DNA. This action facilitates efficient gene expression and is important in maintaining proper cellular functions and responses to environmental signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nIWS1 integrates into critical cellular processes like the cell cycle and DNA damage response pathways. One significant pathway is the RNA polymerase II transcription elongation pathway where IWS1 works closely with other elongation factors and chromatin remodeling proteins to ensure proper transcription regulation. Additionally IWS1 is associated with the DNA damage response pathway where it collaborates with proteins such as ATR and BRCA1 to manage cellular reactions to DNA damage contributing to genome stability and repair mechanisms.\u003cbr\u003e\nIWS1 has been linked to cancer progression with a particular connection to breast and prostate cancers. The overexpression of IWS1 can lead to uncontrolled cell proliferation a feature observed in tumor cells. IWS1 has associations with the protein c-Myc which is a well-known oncogene involved in these cancers. Additionally IWS1's dysregulation may influence the pathogenesis of other proliferative disorders due to its role in transcription control and cellular growth processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855778926761,"sku":"ab263237","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263237","provider":"Iright","version":"1.0","type":"link"}