{"product_id":"abcam-ab263252","title":"Abcam, ab263252, Human MISP (C19orf21) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nMISP KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MISP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nC19orf21 also known as Chromosome 19 Open Reading Frame 21 is a protein with a molecular mass of approximately 26 kDa. It is expressed in various tissues with significant activity in the spleen and lung. Mechanically C19orf21 functions in nuclear processes including transcription regulation and chromatin remodeling. Due to its involvement in these activities C19orf21 interacts with nuclear components and may impact gene expression by influencing chromatin structure.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nC19orf21 plays a role in regulating cellular homeostasis and proliferation. C19orf21 is part of the chromatin remodeling complex which modifies chromatin to allow access to transcription machinery. This participation suggests importance in controlling gene accessibility. C19orf21's involvement in such intricate processes indicates a possible role in cellular development and differentiation influencing tissue-specific gene expression.\u003cbr\u003e\nPathways\u003cbr\u003e\nC19orf21 is involved in chromatin modification and transcriptional regulation pathways. It interacts with proteins like histone acetyltransferases contributing to epigenetic modifications and regulation of gene expression. Additionally C19orf21 shows involvement in pathways that maintain DNA integrity working alongside proteins like DNA repair enzymes. These interactions suggest that C19orf21 supports cellular responses to damage and stress.\u003cbr\u003e\nC19orf21 has been implicated in certain types of cancer and neurological disorders. In cancer it relates to transcription dysregulation potentially interacting with oncogenic factors. Aberrant expression or mutation of C19orf21 may disrupt normal gene regulation leading to tumorigenesis. In neurological disorders C19orf21's role in chromatin remodeling could affect gene expression patterns associated with neural function linking it to conditions such as Alzheimer's disease. In these contexts C19orf21 may interact with known proteins involved such as beta-amyloid precursor protein in Alzheimer's suggesting a wider implication in disease mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845188407465,"sku":"ab263252","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263252","provider":"Iright","version":"1.0","type":"link"}