{"product_id":"abcam-ab263257","title":"Abcam, ab263257, Human MRPL2 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nMRPL2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 100 bp deletion in exon1 and 43 bp insertion in exon1 and 7 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 100 bp deletion in exon1 and 43 bp insertion in exon1 and 7 bp deletion in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MRPL2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMRPL2 also known as mitochondrial ribosomal protein L2 plays an important role in protein synthesis within the mitochondria. It forms a component of the large subunit of the mitochondrial ribosome which has a mass approximately around 35 kDa. This protein consists of a structural framework necessary for the ribosome to translate mitochondrial mRNA into functional proteins. MRPL2 is mainly expressed in tissues with high energy demands including the heart and skeletal muscles and is considered an essential protein for mitochondrial function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMRPL2 contributes to efficient protein manufacturing within the mitochondria by being a part of the mitochondrial ribosomal complex. This complex is responsible for translating 13 essential polypeptides that integrate into the inner mitochondrial membrane and are important for oxidative phosphorylation. MRPL2's presence ensures the translation process occurs smoothly directly influencing cellular energy production. The integrity of mitochondrial protein synthesis significantly affects overall cellular metabolism and energy balance.\u003cbr\u003e\nPathways\u003cbr\u003e\nMRPL2 influences mitochondrial translation and oxidative phosphorylation. It works within pathways that maintain cellular energy production directly impacting cell survival and function. During these processes MRPL2 interacts with essential components like mitochondrial ATP synthase and cytochrome c oxidase facilitating energy conversion from food into usable ATP. These close interactions demonstrate MRPL2's importance in sustaining energy homeostasis and cellular respiration.\u003cbr\u003e\nMRPL2 mutations or dysfunctions have been linked to mitochondrial disorders particularly those affecting muscle and neural tissues such as mitochondrial myopathy. These disorders often manifest due to disrupted mitochondrial protein synthesis leading to defective energy production. In the context of these disorders MRPL2 interacts closely with proteins like NADH dehydrogenase where disruption often results in impaired electron transport and energy conversion. The understanding of MRPL2 in mitochondrial function provides insights into metabolic diseases and can potentially guide therapeutic interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845182148777,"sku":"ab263257","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263257","provider":"Iright","version":"1.0","type":"link"}