{"product_id":"abcam-ab263258","title":"Abcam, ab263258, Human MRPL50 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nMRPL50 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 7 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 7 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MRPL50, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMRPL50 also known as Mitochondrial Ribosomal Protein L50 is a component of the mitochondrial ribosome. With a molecular mass of approximately 16.5 kDa MRPL50 is located within the mitochondria the energy-producing centers of the cell. It forms part of the large 39S subunit of mitochondrial ribosomes and is essential for protein synthesis within mitochondria. Expression of MRPL50 occurs in tissues with high energy demands like muscle and brain reflecting its role in mitochondrial function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMRPL50 plays an essential role in the formation and function of the mitochondrial ribosomal large subunit. A component of the mitochondrial ribosomal complex it contributes to translating mitochondrial mRNA into proteins necessary for oxidative phosphorylation. This function is critical for the maintenance of mitochondrial activity and by extension cellular energy metabolism. The interactions within the ribosomal complex help ensure the efficient synthesis of proteins encoded by mitochondrial DNA.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe function of MRPL50 integrates into the oxidative phosphorylation and mitochondrial translation pathways. This protein is important for the synthesis of components in the electron transport chain a pivotal pathway in energy production. MRPL50 operates together with other mitochondrial ribosomal proteins in translating mitochondrial genes into functional proteins which link into pathways like those involving the NADH dehydrogenase complex.\u003cbr\u003e\nMalfunctions or mutations in MRPL50 associate with mitochondrial diseases such as mitochondrial myopathy and neurodegenerative disorders. The role of MRPL50 in producing mitochondrial proteins links it to conditions where mitochondrial energy production is compromised. Related proteins in these diseases include those involved in the assembly and stability of the mitochondrial ribosomes as well as key constituents of the oxidative phosphorylation system.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847387500713,"sku":"ab263258","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263258","provider":"Iright","version":"1.0","type":"link"}