{"product_id":"abcam-ab263259","title":"Abcam, ab263259, Human MRPS28 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nMRPS28 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MRPS28, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMRPS28 also known as Mitochondrial Ribosomal Protein S28 is a protein that plays an important role in mitochondrial protein synthesis. It is a component of the small subunit of the mitochondrial ribosome which is important for the translation of mitochondrial mRNAs. This protein has a molecular mass of approximately 12 kDa and is mainly expressed in the mitochondria an organelle present in most eukaryotic cells. As a mitochondrial ribosomal protein MRPS28 is part of the machinery responsible for producing proteins that are essential for mitochondrial function and energy production.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMRPS28 is involved in the assembly and stability of the mitochondrial ribosomal small subunit complex. It ensures the proper translation of 13 mitochondrial-encoded polypeptides which are components of the oxidative phosphorylation system. MRPS28 interacts with other mitochondrial ribosomal proteins to form a functional ribosome supporting mitochondrial gene expression and cellular energy metabolism. The presence of MRPS28 in this complex is necessary for the maintenance of mitochondrial translation fidelity and efficiency.\u003cbr\u003e\nPathways\u003cbr\u003e\nMRPS28 plays a role in mitochondrial translation and oxidative phosphorylation pathways. It contributes to the synthesis of proteins that are integral to the electron transport chain an important part of energy production in cells. Proteins such as MRPS16 and MRPS18 are also involved within these same pathways working closely with MRPS28 to ensure efficient mitochondrial function. These pathways are essential to provide energy in the form of ATP supporting various cellular processes.\u003cbr\u003e\nMRPS28 has connections to conditions associated with mitochondrial dysfunction such as mitochondrial myopathy and Leigh syndrome. Research has suggested that mutations affecting MRPS28 could impair mitochondrial translation leading to decreased energy production and resulting in these disorders. Through its role in these diseases MRPS28 is linked with other mitochondrial proteins like MRPL3 which when dysfunctional can also contribute to similar mitochondrial pathologies. Understanding the function and disease associations of MRPS28 may provide insights into potential therapeutic strategies for mitochondrial diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845071065257,"sku":"ab263259","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263259","provider":"Iright","version":"1.0","type":"link"}