{"product_id":"abcam-ab263267","title":"Abcam, ab263267, Human MVB12A (FAM125A) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nMVB12A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1 and 40 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon1 and 40 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MVB12A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFAM125A also known as MVB12A is a protein involved in the endosomal sorting complex required for transport particularly the ESCRT-I complex. It is about 26 kDa in mass and plays a role in cargo recognition during the sorting process. FAM125A is expressed in many tissues including the central nervous system indicating its wide functional relevance.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFAM125A participates in the ESCRT machinery which is essential for the regulation of endosomal trafficking. It associates with other proteins such as TSG101 within the ESCRT-I complex facilitating the sorting of ubiquitinated membrane proteins into multivesicular bodies. This activity is vital for processes like receptor down-regulation and virus budding indicating its significant involvement in cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nFAM125A significantly engages in the endocytic and lysosomal degradation pathways. It works closely with ESCRT-I complex partners such as VPS37 and TSG101 aiding in membrane remodeling and protein trafficking. The ESCRT pathway is important for maintaining proper cell signaling and membrane composition linking FAM125A functionally to cellular maintenance and response processes.\u003cbr\u003e\nFAM125A has associations with neurogenerative conditions and certain cancers. Disrupted endosomal sorting might contribute to pathological states where protein aggregation or receptor recycling is impaired such as in Alzheimer's disease. Additionally FAM125A and ESCRT-related proteins like VPS37 may play roles in tumor progression and metastasis as proper endosome function is necessary to control cell division and migration signals.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845174710441,"sku":"ab263267","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263267","provider":"Iright","version":"1.0","type":"link"}