{"product_id":"abcam-ab263319","title":"Abcam, ab263319, Human PTPRF (LAR) knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPTPRF KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 4 and 5 bp deletion in exon 4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 4 and 5 bp deletion in exon 4.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PTPRF, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe protein LAR also known as Leukocyte Common Antigen-Related phosphatase is a receptor-like protein tyrosine phosphatase. It weighs approximately 220 kDa. LAR is expressed in various tissues including the nervous system and digestive tract. It comprises an extracellular domain a single transmembrane domain and two intracellular phosphatase domains which play a significant role in its interaction with other proteins.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis receptor-like phosphatase supports important cell signaling functions. LAR is involved in neural development processes and is part of the synaptic adhesion complex. By interacting with proteins in the plasma membrane it helps modify and maintain neural connectivity necessary for proper nerve function. Its activity influences actin cytoskeleton dynamics and cell-cell adhesion.\u003cbr\u003e\nPathways\u003cbr\u003e\nLAR plays a role in the axon guidance and insulin signaling pathways. In axon guidance it interacts with proteins such as netrin and nneural cell adhesion molecule (NCAM) to facilitate correct neural wiring. In the insulin signaling pathway LAR can dephosphorylate key proteins like insulin receptor affecting glucose metabolism and overall energy homeostasis.\u003cbr\u003e\nResearch shows that LAR may be linked to diabetes and potential neurological disorders. In diabetes its interaction with the insulin receptor suggests a role in insulin resistance which is a major contributing factor in the disease. In neurological disorders LAR’s dysregulation can affect neural development and function possibly linking it to diseases like schizophrenia where cell signaling and connectivity changes are observed.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845179986089,"sku":"ab263319","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263319","provider":"Iright","version":"1.0","type":"link"}