{"product_id":"abcam-ab263321","title":"Abcam, ab263321, Human PTPRS knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPTPRS KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon5 and 1 bp insertion in exon5.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon5 and 1 bp insertion in exon5.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PTPRS, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPTPRS also known as Protein Tyrosine Phosphatase Receptor Type S is an enzyme with a molecular mass of approximately 193 kDa. It operates as a receptor-type protein tyrosine phosphatase located on the cell surface. The protein consists of an extracellular domain a single transmembrane segment and an intracellular catalytic domain. Expression of PTPRS occurs in various tissues with significant presence in the central nervous system. It plays a role in cell signaling by dephosphorylating specific phosphotyrosine residues influencing cellular communication and signal transduction.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nProtein Tyrosine Phosphatase Receptor Type S affects multiple cellular processes. It is not part of a large protein complex but interacts with cell adhesion molecules and other proteins involved in cell growth and differentiation. PTPRS contributes to neural development and repair by influencing axonal growth and guidance. It regulates synaptic architecture by modulating signaling pathways that affect neural plasticity and regeneration.\u003cbr\u003e\nPathways\u003cbr\u003e\nPTPRS is involved in neural-related signaling cascades modulating cellular responses. One notable pathway where PTPRS plays a significant role is the Src pathway which regulates processes related to growth and differentiation. PTPRS interacts with proteins like Src kinase family members impacting their activity through dephosphorylation. This modulation of kinase activity directly influences cellular outcomes such as proliferation and cell motility.\u003cbr\u003e\nProtein Tyrosine Phosphatase Receptor Type S shows connections to neurodegenerative conditions and cancer. Altered PTPRS function associates with Alzheimer's disease where disrupted signaling affects neural pathways and contributes to the disease pathogenesis. In certain cancers abnormal PTPRS expression influences tumor progression by affecting cellular adhesion and migration. The protein interacts with cancer-related proteins such as integrins further contributing to disease development and progression through its regulatory mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845175333033,"sku":"ab263321","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263321","provider":"Iright","version":"1.0","type":"link"}