{"product_id":"abcam-ab263327","title":"Abcam, ab263327, Human REXO4 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nREXO4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-REXO4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nREXO4 also known as RNA exonuclease homolog 4 is a protein that plays a role in RNA processing. It has a molecular mass of approximately 33 kDa. REXO4 is found in the nucleolus and also distributes within the cytoplasm. This protein exhibits exonuclease activity participating in the degradation and\/or processing of RNA molecules. By cutting RNA molecules it facilitates the regulation of gene expression which is important for proper cellular function and growth.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRNA exonucleases like REXO4 contribute to RNA surveillance processes that maintain the quality of RNA. REXO4 might function as part of a larger complex tasked with the maturation and degradation of RNA. These activities are essential especially in the context of removing faulty RNA molecules that could cause deleterious effects if left unchecked in the cell. The precise role of REXO4 remains under investigation yet its contributions to the RNA life cycle are noteworthy.\u003cbr\u003e\nPathways\u003cbr\u003e\nRNA processing and degradation involve REXO4 in essential molecular interactions. It participates in the RNA degradation pathway where it contributes to the turnover of RNA molecules ensuring cellular stability and homeostasis. This pathway often intersects with the nonsense-mediated decay pathway a critical quality control mechanism. Proteins such as UPF1 which are involved in the nonsense-mediated decay pathway might be functionally connected to REXO4 highlighting its importance in maintaining cellular RNA integrity.\u003cbr\u003e\nDefects or dysregulation in the function of REXO4 may contribute to RNA-related conditions. Aberrant RNA processing has connections to disorders such as cancer. In certain cancers malfunctioning RNA degradation can lead to uncontrolled cell proliferation. Moreover faulty REXO4 activity might impact neurodegenerative diseases by affecting neuronal RNA metabolism. Interactions with proteins like TARDBP associated with such neurodegenerative disorders suggest that REXO4 may have broader implications in understanding RNA-linked diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845192110249,"sku":"ab263327","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263327","provider":"Iright","version":"1.0","type":"link"}