{"product_id":"abcam-ab263330","title":"Abcam, ab263330, Human RNF149 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRNF149 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RNF149, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRNF149 also known as RING Finger Protein 149 is an E3 ubiquitin-protein ligase which means it catalyzes the transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate protein tagging it for degradation. The mass of RNF149 is approximately 49 kDa. It can be found expressed in various tissues with notable presence in the liver and kidney. This protein contains a RING-type zinc finger domain which is critical for its ligase activity and substrate specificity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRNF149 participates in protein quality control by mediating the ubiquitination of proteins that require degradation therefore maintaining protein homeostasis. It acts independently and is not part of a larger protein complex. By tagging damaged or misfolded proteins for degradation RNF149 contributes to the cellular stress response ensuring that cells respond to potential stressors effectively and efficiently.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of RNF149 in the ubiquitin-proteasome system places it within the protein degradation pathway which is essential for regulating protein turnover and cellular function. It interacts with several other proteins including ubiquitin-conjugating enzyme E2s in this pathway. RNF149 may also intersect with apoptosis pathways due to its role in controlling the degradation of apoptotic regulators linking it to proteins involved in cell death processes.\u003cbr\u003e\nRNF149 shows a connection to certain cancers and neurodegenerative diseases. Abnormal expression or mutations in RNF149 can relate to the deregulation of protein turnover contributing to the development of hepatocellular carcinoma. It is also connected to neurodegenerative disorders due to its role in degrading damaged proteins with connections to proteins like p53 in cancer pathways and tau proteins in neurological contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845128769705,"sku":"ab263330","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263330","provider":"Iright","version":"1.0","type":"link"}