{"product_id":"abcam-ab263332","title":"Abcam, ab263332, Human RPRM (Protein reprimo) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRPRM KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 106 bp deletion in exon1 and 53 bp deletion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 106 bp deletion in exon1 and 53 bp deletion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RPRM, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nProtein reprimo (RPRM) also known as Reprimo is a small protein with a molecular mass of approximately 12.5 kDa. It does its mechanical function by regulating cell cycle arrest specifically at the G2\/M checkpoint. RPRM localizes to the cytoplasm and is expressed in a varied range of tissues including the stomach and colon indicating its importance in gastrointestinal biology. It interacts with the cytoskeletal elements to exert its influence on cellular activities.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRPRM acts as a tumor suppressor by inhibiting cell proliferation mainly through its G2 arrest function. It is not formally known to be part of a larger protein complex but it works closely with p53 a well-known regulatory protein and tumor suppressor to mediate its effects. RPRM expression helps prevent abnormal cell growth under stress conditions contributing to the maintenance of genomic stability.\u003cbr\u003e\nPathways\u003cbr\u003e\nRPRM plays critical roles in the p53 signaling pathway and the cell cycle control pathway. In the p53 pathway RPRM aids in enforcing a cell cycle halt when DNA damage is detected working alongside other proteins such as p21 and GADD45. In the context of cell cycle control RPRM functions as an intermediary that facilitates the arrest of cell division in response to DNA stress signals collaborating with these proteins to enhance DNA repair mechanisms.\u003cbr\u003e\nRPRM interactions significantly impact cancer particularly gastric cancer and colorectal cancer. The protein's reduced expression is frequently seen in malignancies where p53 pathway disruptions are apparent. In these cancers connections exist between the downregulation of RPRM and overexpression of Bcl-2 an anti-apoptotic protein where RPRM loss results in impaired apoptosis and uncontrolled cell survival. This association marks RPRM as a potential biomarker for understanding tumor dynamics in these types of cancers.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845175496873,"sku":"ab263332","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263332","provider":"Iright","version":"1.0","type":"link"}