{"product_id":"abcam-ab263336","title":"Abcam, ab263336, Human RTN4RL2 (NgR2) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRTN4RL2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RTN4RL2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNgR2 also known as Nogo receptor 2 or Reticulon 4 receptor 2 is a glycosylphosphatidylinositol (GPI)-anchored protein with a molecular mass of approximately 55 kDa. It is mainly expressed in the central nervous system where it functions as an axon guidance receptor. NgR2 binds to myelin-associated proteins including myelin-associated glycoprotein (MAG) oligodendrocyte myelin glycoprotein (OMgp) and Nogo-A inhibiting axon regeneration in the adult nervous system. This protein is important for maintaining neuronal architecture and regulating neuronal growth.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe action of NgR2 impacts the neural regeneration processes by forming a receptor complex with p75 neurotrophin receptor (p75NTR) and LINGO-1. This complex activation inhibits axonal outgrowth and neural plasticity which affects the nervous system's ability to recover after injury. NgR2 mediates signaling that prevents neurite outgrowth contributing to the stabilization of neuronal connections in postnatal brain development. NgR2's role in these processes makes it a significant target for studies investigating neuronal repair mechanisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nAxon guidance and myelin inhibition play significant roles in the function of NgR2. It participates in the RhoA-ROCK signaling pathway which is activated upon myelin ligand binding to the receptor complex. This pathway is responsible for cytoskeletal changes that inhibit neurite extension. Additionally NgR2 interacts with proteins such as Nogo-A and MAG further influencing axonal growth inhibition and regeneration in the nervous system.\u003cbr\u003e\nDefects in NgR2 function or expression can relate to conditions such as spinal cord injury and multiple sclerosis. These disorders exhibit impaired neural repair and regeneration where NgR2 and its associated proteins like Nogo-A and MAG play a role in limiting recovery and repair mechanisms. Understanding the interactions and regulation of NgR2 in these contexts can provide insights into therapeutic approaches aimed at enhancing neural regeneration and functional recovery.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855783088297,"sku":"ab263336","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263336","provider":"Iright","version":"1.0","type":"link"}