{"product_id":"abcam-ab263348","title":"Abcam, ab263348, Human SLC12A9 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSLC12A9 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SLC12A9, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSLC12A9 also known as CCC9 is part of the cation-chloride cotransporter family. It is a protein with an approximate mass of 120 kDa. This transporter is involved in moving ions across cell membranes particularly sodium potassium and chloride ions playing a significant role in maintaining cellular homeostasis. SLC12A9 is expressed in various tissues including the kidney and brain suggesting its importance across different physiological systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe protein facilitates ion transport within cells contributing to osmoregulation and volume control. Although SLC12A9 is not a central part of any known large protein complex its ion transport functionality indicates it might interact with other membrane proteins involved in similar regulatory roles. This interaction helps modulate cell volume and osmolality which influences cell survival and function.\u003cbr\u003e\nPathways\u003cbr\u003e\nSLC12A9 plays a role in the cellular signaling and homeostasis pathways. It is particularly associated with the WNK (With No Lysine) kinases-pathway which regulates electrolyte balance and blood pressure. In this context SLC12A9 has relations with other transporters like NKCC1 and KCC2 which also participate in the same ion transport and regulatory processes indicating cooperative interactions in maintaining cellular ionic balance.\u003cbr\u003e\nSeveral studies have linked SLC12A9 to hypertension due to its role in regulating blood pressure via ion transport. Additionally research suggests its potential involvement in neurological disorders given its expression in brain tissues. Mutations or dysregulation of SLC12A9 could affect other related proteins such as WNK kinases contributing to these pathologies by altering ion transport dynamics and impacting overall cellular function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847387533481,"sku":"ab263348","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263348","provider":"Iright","version":"1.0","type":"link"}