{"product_id":"abcam-ab263365","title":"Abcam, ab263365, Human SSBP3 knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nSSBP3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 101 bp deletion in exon3.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 101 bp deletion in exon3.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SSBP3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSingle-stranded DNA-binding protein 3 (SSBP3) functions mechanically by binding to single-stranded DNA stabilizing it and facilitating replication and repair processes. It is sometimes referred to as SSBP4 due to similarities with closely related proteins. SSBP3 has a molecular weight of around 33 kDa. It expresses in various tissues with higher expression levels in the liver kidney and brain. This protein plays a role in the regulation of DNA metabolic processes through its interaction with single-stranded DNA.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSSBP3 participates in DNA replication recombination and repair mechanisms. The protein acts as a part of a multi-protein complex that includes the replication protein A (RPA) which is essential for maintaining genomic stability. This association helps in the recruitment of other proteins needed for proper DNA metabolic activities. By stabilizing single-stranded DNA SSBP3 contributes to efficient DNA processing ensuring accurate cellular function and integrity.\u003cbr\u003e\nPathways\u003cbr\u003e\nSingle-stranded DNA-binding protein 3 plays a role in the DNA damage repair pathway and the DNA replication pathway. It associates closely with proteins such as replication protein A (RPA) and the ataxia-telangiectasia mutated (ATM) protein which are significant in responding to DNA damage and ensuring proper DNA replication. Through these interactions SSBP3 influences DNA repair mechanisms and cell cycle progression protecting cells from genotoxic stress.\u003cbr\u003e\nSSBP3 has links to neurodegenerative disorders and certain cancers. Alterations in the expression or function of SSBP3 may contribute to genomic instability seen in these conditions. Specifically its association with the ATM protein in the DNA repair pathway connects SSBP3 to ataxia-telangiectasia a disorder characterized by neurodegeneration and increased cancer risk. Research continues to explore how SSBP3’s role in DNA stabilization and repair affects these disease processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845191159977,"sku":"ab263365","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263365","provider":"Iright","version":"1.0","type":"link"}