{"product_id":"abcam-ab263387","title":"Abcam, ab263387, Human TMEM126A knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTMEM126A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TMEM126A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTMEM126A also known as Transmembrane Protein 126A is a mitochondrial protein with an approximate mass of 26 kDa. This protein is integral to the mitochondrial inner membrane where it plays a role in maintaining mitochondrial function. TMEM126A is expressed in many tissues but shows high levels in energy-demanding tissues like heart and skeletal muscle.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTMEM126A contributes to the stability and assembly of the mitochondrial respiratory chain Complex I. It forms part of the mitochondrial contact site and cristae organizing system (MICOS) essential for maintaining the inner membrane architecture. By assisting in Complex I assembly TMEM126A supports efficient cellular respiration and energy production which is critical for cell survival and activity.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe role of TMEM126A is significant in oxidative phosphorylation and mitochondrial respiration pathways. It interacts closely with Complex I components like NDUFAF1 and NDUFS1. These interactions are vital for proper electron transport chain function impacting cellular energy homeostasis and metabolic regulation.\u003cbr\u003e\nTMEM126A mutations relate to mitochondrial complex I deficiency and optic atrophy type 7. Complex I deficiency affects energy production leading to neurological and muscular diseases. In optic atrophy type 7 faulty mitochondrial function from TMEM126A links with vision loss. TMEM126A works alongside proteins such as OPA1 and OXPHOS components in these conditions highlighting its importance in mitochondrial health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845183033513,"sku":"ab263387","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263387","provider":"Iright","version":"1.0","type":"link"}