{"product_id":"abcam-ab263389","title":"Abcam, ab263389, Human TMEM140 knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTMEM140 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2 and 4 bp deletion in exon2 and 8 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2 and 4 bp deletion in exon2 and 8 bp deletion in exon2.,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TMEM140, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTMEM140 also known as Transmembrane Protein 140 is a membrane protein with an approximate mass of 31 kDa. This protein spans the cell membrane and currently researchers identify its presence in various tissues including the brain and testis. TMEM140 involves in cellular signal transduction due to its integral membrane position and though the precise mechanical action remains under exploration experts suspect it plays a part in intracellular communication.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTMEM140 impacts cell processes involving cellular differentiation and development. The protein does not traditionally belong to any larger multiprotein complex but preliminary evidence suggests it may interact with specific signaling molecules. Its expression and function imply potential roles in neurogenesis and gametogenesis contributing to the broader understanding of development.\u003cbr\u003e\nPathways\u003cbr\u003e\nTMEM140 connects with pathways involving neural development and reproductive processes. It appears to interact with proteins related to these pathways which include other transmembrane proteins integral to cellular signaling. Experts study its involvement within MAPK signaling cascades a pathway important for transmitting extracellular signals to the cell nucleus affecting processes like growth and differentiation.\u003cbr\u003e\nAlterations in TMEM140 function link to neurodegenerative disorders like Alzheimer's disease and certain fertility issues. The protein interacts with proteins such as tau in neuronal contexts which relates to neurodegeneration. In reproductive health aberrant TMEM140 expression might affect proteins in the spermatogenesis pathway potentially leading to infertility.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845185261737,"sku":"ab263389","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263389","provider":"Iright","version":"1.0","type":"link"}