{"product_id":"abcam-ab263404","title":"Abcam, ab263404, Human TXNRD3 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTXNRD3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon9 and 1 bp insertion in exon9.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon9 and 1 bp insertion in exon9.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TXNRD3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTXNRD3 also known as thioredoxin reductase 3 is an enzyme that plays a role in reducing thioredoxin proteins using NADPH as a cofactor. It typically exists as a homodimer and carries a mass of approximately 70 kDa per subunit. TXNRD3 is located in diverse tissues including testis and various other organs reflecting its versatile role in metabolism and cellular defense against oxidative stress.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme functions to maintain a reduced cellular environment by reducing thioredoxin which in turn affects other cellular proteins. TXNRD3 is part of the thioredoxin system a complex that includes thioredoxin and NADPH which are essential for redox homeostasis and cellular defense mechanisms. It drives the reduction of oxidized forms of biomolecules supporting processes like DNA synthesis and repair.\u003cbr\u003e\nPathways\u003cbr\u003e\nTXNRD3 significantly contributes to redox signaling and antioxidant defense. It has a pivotal position in the cellular antioxidative defense pathway by working closely with glutaredoxin and thioredoxin systems. TXNRD3 interacts with proteins such as thioredoxin helping regulate cellular redox states and protect cells from oxidative damage through these pathways. These interactions are critical for maintaining cellular functions under oxidative stress conditions.\u003cbr\u003e\nTXNRD3 has connections with conditions involving oxidative stress and redox imbalance such as cancer. Alterations in TXNRD3 expression or activity might lead to dysregulation of redox homeostasis. In cancer the enzyme's relationship with thioredoxin and other redox-regulating proteins plays a part in tumorigenesis. Understanding TXNRD3's roles provides valuable insight into the development and progression of disease and emphasizes potential therapeutic targets for intervention.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845129293993,"sku":"ab263404","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263404","provider":"Iright","version":"1.0","type":"link"}