{"product_id":"abcam-ab263426","title":"Abcam, ab263426, Human YIF1A knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nYIF1A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-YIF1A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nYIF1A also known as Yip1 Family Member 1 or Yin Yang 1-Interacting Factor 1 functions mechanically as a membrane protein involved in trafficking between the endoplasmic reticulum (ER) and the Golgi apparatus. It has a molecular mass of approximately 35 kDa. This protein localizes predominantly to intracellular membranous structures most notably within the ER and the Golgi. Its expression occurs in various tissues including brain liver and kidney where it plays specialized roles in protein transport and cellular homeostasis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein interacts with various components of intracellular transport machinery. YIF1A is part of a larger YIP1 complex that supports vesicle formation and trafficking essential for maintaining proper cellular function. Through its interaction with this complex YIF1A contributes to the regulation of protein sorting and distribution which is necessary for functional membrane dynamics and signal transduction.\u003cbr\u003e\nPathways\u003cbr\u003e\nYIF1A participates in intracellular transport and vesicle-mediated processes. It associates with the vesicular trafficking pathway where it regulates the movement and sorting of proteins between the ER and Golgi. YIF1A works in conjunction with proteins like YIPF5 in these pathways which coordinate the accurate delivery and modification of proteins necessary for normal cellular operations.\u003cbr\u003e\nYIF1A's dysregulation links to neurodegenerative diseases such as Parkinson's disease. Misfunction in YIF1A can lead to improper protein trafficking contributing to cellular stress and neuron degeneration. Additionally YIF1A connects to the sigma-1 receptor a protein implicated in amyotrophic lateral sclerosis (ALS) indicating its relevance in neurological health. Understanding these associations can offer insights into potential therapeutic targets for these ailments.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855784464553,"sku":"ab263426","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab263426","provider":"Iright","version":"1.0","type":"link"}