{"product_id":"abcam-ab264722","title":"Abcam, ab264722, Human MC2R knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nMC2R KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MC2R, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe melanocortin 2 receptor (MC2-R) also known as ACTH receptor is a protein that mechanically functions as a G protein-coupled receptor (GPCR). It is responsible for mediating activity through adenylate cyclase signaling by binding with adrenocorticotropic hormone (ACTH). This receptor has a molecular mass of approximately 33 kDa. MC2-R is mostly expressed in the adrenal cortex where it plays a role in synthesizing and releasing glucocorticoids.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe action of MC2-R initiates the adrenal response to stress through glucocorticoid production which is important for maintaining homeostasis in the body. MC2-R functions as a standalone entity rather than as part of a larger complex. By binding to ACTH MC2-R triggers downstream signaling that leads to steroidogenesis. The biosynthesis of glucocorticoids helps in managing energy immune function and circadian rhythm.\u003cbr\u003e\nPathways\u003cbr\u003e\nMC2-R is integral in the cAMP signaling pathway and the stress response pathway. In the cAMP signaling activation of MC2-R by ACTH results in increased production of cyclic AMP further promoting enzyme activation essential for glucocorticoid synthesis. Its activity is connected to the broader steroid hormone biosynthesis pathway working closely with proteins like StAR (steroidogenic acute regulatory protein) which facilitates cholesterol transport to the inner mitochondrial membrane.\u003cbr\u003e\nDisruptions in MC2-R function can lead to conditions like familial glucocorticoid deficiency and Cushing's syndrome. Dysfunctional receptor signaling may lead to inadequate glucocorticoid production or excessive hormone levels. The role of MC2-R in these disorders connects it closely with proteins like MC1-R another melanocortin receptor involved in pigmentation and inflammatory response which also signifies related signaling cascade abnormalities.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845153935529,"sku":"ab264722","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab264722","provider":"Iright","version":"1.0","type":"link"}