{"product_id":"abcam-ab264753","title":"Abcam, ab264753, Human RRAGC knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nRRAGC KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RRAGC, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe RRAGC protein also known as Ras-related GTP-binding protein C weighs approximately 44.5 kDa. It functions mechanically as a GTPase cycling between active GTP-bound and inactive GDP-bound states. This protein is expressed in various tissues with noticeable presence in the brain and liver. RRAGC plays a role in cellular signaling and growth mechanisms by interacting with other members of the Ras superfamily.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRRAGC forms a part of the heterodimeric Rag GTPase complex often teaming with its partner RRAGB. This complex acts as an important regulator in the mTORC1 signaling pathway influencing nutrient sensing and energy balance within cells. RRAGC's ability to toggle between different energy states allows it to contribute to cellular metabolism and response to external stress.\u003cbr\u003e\nPathways\u003cbr\u003e\nRRAGC engages directly in the mTOR signaling pathway an important driver of cell growth and proliferation. It interacts closely with related proteins such as Raptor and mTOR kinase forming a bridge between amino acid availability and cellular growth signals. Additionally RRAGC's role connects to the autophagy pathway affecting cellular degradation processes and nutrient recycling under nutrient-deprived conditions.\u003cbr\u003e\nThe dysregulation of RRAGC's activity associates with conditions like cancer and neurodegenerative diseases. Alterations in its function may link to aberrant mTOR signaling contributing to uncontrolled cellular proliferation seen in cancers. Furthermore RRAGC and its interactions with proteins such as TSC2 (Tuberous Sclerosis Complex 2) highlight potential influences in neurodegenerative disease pathways shedding light on its involvement in conditions linked to cellular growth dysregulation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845150036137,"sku":"ab264753","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab264753","provider":"Iright","version":"1.0","type":"link"}