{"product_id":"abcam-ab264819","title":"Abcam, ab264819, Human ITGA2 (CD49b) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nITGA2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 14 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 14 bp deletion in exon 4,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ITGA2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCD49b also known as Integrin alpha-2 or ITGA2 is a transmembrane glycoprotein with a molecular weight of approximately 160 kDa. This protein localizes on the surface of various cells including platelets fibroblasts and some immune cells. It functions as part of the integrin family which are receptors that mediate the cell-extracellular matrix (ECM) interactions. CD49b binds to collagen and laminin in the ECM and contributes to cellular adhesion and signal transduction.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe CD49b protein plays a critical role in cell migration thrombosis and wound healing. By forming a heterodimer with Integrin beta-1 it becomes a part of the collagen receptor complex Integrin alpha-2\/beta-1 which facilitates various cellular interactions with the ECM. This functionality enables cells to sense their environment and respond to mechanical changes which is important for processes such as tissue repair and embryonic development.\u003cbr\u003e\nPathways\u003cbr\u003e\nSeveral cellular mechanisms involve CD49b in maintaining physiological homeostasis. One significant pathway is the integrin signaling pathway where CD49b interacts with focal adhesion kinase (FAK) and other intracellular proteins to regulate cytoskeletal rearrangement and cell motility. Another pathway is platelet activation where CD49b collaborates with glycoprotein VI (GPVI) and other receptors to initiate platelet aggregation during hemostasis. These pathways highlight the importance of CD49b in various cellular functions related to cell mobility and stability.\u003cbr\u003e\nResearchers have linked CD49b to conditions including thrombosis and fibrosis. In thrombosis abnormal platelet aggregation often involves CD49b and its interaction with fibrinogen and von Willebrand factor. In fibrosis CD49b's role in cell adhesion and matrix remodeling correlates with excessive ECM deposition and scar formation. Understanding CD49b's function offers insights into therapeutic strategies alongside related proteins such as Integrin beta-1 which may modulate disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845065658537,"sku":"ab264819","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab264819","provider":"Iright","version":"1.0","type":"link"}