{"product_id":"abcam-ab264907","title":"Abcam, ab264907, Human BAZ1B (WSTF) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nBAZ1B KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 5 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 5 bp deletion in exon 2,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-BAZ1B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nWSTF also known as Williams Syndrome Transcription Factor or BAZ1B is a versatile protein with an estimated mass around 175 kDa. It is a component of the nucleosome remodeling factor (NURF) and the Williams-Beuren syndrome chromosome region 17 (WBSCR17) complex. WSTF has widespread expression in various tissues including the brain and heart which highlights its involvement in diverse cellular processes. Functionally it facilitates chromatin remodeling by altering the structure of nucleosomes which is vital for DNA accessibility in transcription DNA repair and replication.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSince WSTF participates in chromatin structure modulation it plays a considerable role in transcriptional regulation. It is a part of the NURF complex which makes it essential for DNA accessibility and gene expression. The complex interacts with other proteins to allow chromatin to accommodate active transcription by repositioning nucleosomes. WSTF also has a kinase domain that phosphorylates histone H2A. This phosphorylation integrates signals that coordinate transcription and DNA damage repair mechanisms highlighting its multifunctional nature in maintaining genomic stability.\u003cbr\u003e\nPathways\u003cbr\u003e\nWSTF has pivotal roles in the chromatin remodeling and DNA repair pathways. The chromatin remodeling pathway involves WSTF's interaction with the transcription factor complex influencing gene accessibility and expression. WSTF in the DNA repair pathway ensures proper genomic integrity through its association with proteins like BRCA1 highlighting its participation in the cellular response to DNA damage. By engaging with these pathways WSTF contributes to the cell's ability to regulate the genome and respond to damage efficiently.\u003cbr\u003e\nWSTF is significantly connected to Williams-Beuren syndrome and certain cancers. Williams-Beuren syndrome a developmental disorder arises from the deletion of the region on chromosome 7 involving the WSTF gene affecting brain and heart function. In certain cancers aberrant expression or mutations within the WSTF gene are associated with uncontrolled chromatin remodeling contributing to tumorigenesis. Through these disorders WSTF shows its interaction with proteins like SMARCA1 in chromatin remodeling anomalies emphasizing its importance in disease progression and cellular dysfunction.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845080240297,"sku":"ab264907","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab264907","provider":"Iright","version":"1.0","type":"link"}