{"product_id":"abcam-ab265115","title":"Abcam, ab265115, Human DDB2 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nDDB2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 1 and 29 bp deletion in exon 1 and 2 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 1 and 29 bp deletion in exon 1 and 2 bp insertion in exon 1,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-DDB2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDDB2 also known as DNA damage-binding protein 2 plays a mechanical role in the nucleotide excision repair (NER) pathway. It is a part of the UV-damaged DNA-binding protein complex and assists in recognizing and cutting damaged DNA. The mass of DDB2 is approximately 48 kDa. The expression of DDB2 occurs broadly but it shows enhanced expression in tissues exposed to UV light like skin.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEngagement in DNA repair mechanisms allows DDB2 to help maintain genomic stability. It forms a critical part of the DDB1-DDB2 complex which collaborates with other proteins in the initial damage recognition step of NER. This involvement ensures the repair of UV-induced damage and bulky DNA adducts preventing mutations that can cause harmful genetic alterations.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of DDB2 is important within the nucleotide excision repair and transcription-coupled repair pathways. In these pathways DDB2 partners with proteins such as XPC and XPA facilitating the recognition and verification of DNA damage. These interactions ensure an accurate and efficient repair process protecting cells from potential genotoxic stress.\u003cbr\u003e\nDDB2 has relevance to skin cancer and xeroderma pigmentosum. Among these mutations or dysregulation involving DDB2 can lead to skin cancer due to impaired DNA repair response to UV damage. It connects to p53 a protein that regulates the cell cycle and likewise contributes to cellular responses against DNA damage. In xeroderma pigmentosum defects in components of the NER pathway including DDB2 lead to extreme sensitivity to sunlight and subsequent skin abnormalities.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845078864041,"sku":"ab265115","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab265115","provider":"Iright","version":"1.0","type":"link"}