{"product_id":"abcam-ab265298","title":"Abcam, ab265298, Human PACSIN2 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nPACSIN2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 3 and 8 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 3 and 8 bp deletion in exon 3,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PACSIN2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPACSIN2 also known as Protein kinase C and casein kinase substrate in neurons protein 2 is a protein encoded by the PACSIN2 gene. This protein has a mass of approximately 54 kDa and functions primarily in the cytoplasm. PACSIN2 is expressed in various tissues with notable expression in the brain and kidney. This protein plays a role in skeletal muscle regulation by interacting with actin and promoting changes in cell shape. PACSIN2 also contributes to the process of endocytosis particularly in coordinating the assembly of proteins that form vesicles.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPACSIN2 regulates cytoskeletal dynamics and membrane trafficking. It acts as a part of a protein complex that includes dynamin which is essential for the fission of vesicular membranes from the plasma membrane during endocytosis. Through its involvement in these processes PACSIN2 assists in maintaining cellular morphology and transport mechanisms within cells. Its interaction with dynamin and actin indicates its role in modulating cellular infrastructure during response to environmental changes or cellular signals.\u003cbr\u003e\nPathways\u003cbr\u003e\nPACSIN2 participates in the endocytic pathway and the regulation of cytoskeletal dynamics. It interacts with proteins like dynamin important in vesicle scission during endocytosis. Moreover PACSIN2 influences pathways related to actin dynamics and cellular morphology changes interacting with actin to coordinate cytoskeletal rearrangements. This involvement highlights its function in cellular adaptation to different stimuli.\u003cbr\u003e\nPACSIN2 is associated with neurodegenerative diseases and cancer. Research has shown its potential involvement in Alzheimer's disease due to its role in membrane trafficking and signaling pathways. In the context of cancer its expression may correlate with abnormal cell proliferation and tumor progression. The interaction between PACSIN2 and dynamin further links it to these disorders as alterations in endocytosis and signaling can affect disease progression and cell growth.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845150003369,"sku":"ab265298","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab265298","provider":"Iright","version":"1.0","type":"link"}