{"product_id":"abcam-ab265307","title":"Abcam, ab265307, Human RQCD1 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nCNOT9 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon 1 and 1 bp insertion in exon 1 and 68 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon 1 and 1 bp insertion in exon 1 and 68 bp deletion in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-CNOT9, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRQCD1 also known as Required for Cell Differentiation 1 is a protein with an approximate mass of 34 kDa. It plays an essential role in gene expression regulation by acting as a transcriptional cofactor. RQCD1 is highly expressed in various tissues including the liver kidney and heart. The protein interacts with different transcription factors and modulates their activities which influences cellular pathways critical for cell development and differentiation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe protein RQCD1 acts as a component of the human mRNA methyltransferase-associated complex. The complex is involved in post-transcriptional modifications. Through its interaction in the complex RQCD1 contributes to RNA processing and stability further affecting gene expression outcomes. This involvement impacts cell cycle regulation and proliferation necessary for maintaining cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe protein RQCD1 is important in the MAPK signaling pathway and Wnt signaling pathway. In these pathways it interacts with proteins like c-Fos and β-catenin influencing pathways related to cell growth and differentiation. Its presence in these pathways demonstrates the protein's role in signal transduction processes that govern various cellular responses.\u003cbr\u003e\nProteins like RQCD1 have shown associations with cancer and metabolic disorders. Aberrant expression or mutations in RQCD1 are linked to the development and progression of certain cancers due to its role in transcriptional regulation. Additionally this protein has connections with metabolic proteins like AMPK indicating its potential impact on disorders related to metabolic imbalance. These roles suggest that RQCD1 is a significant player in both cancer biology and metabolism-related diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845149151401,"sku":"ab265307","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab265307","provider":"Iright","version":"1.0","type":"link"}