{"product_id":"abcam-ab265309","title":"Abcam, ab265309, Human MT1X knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nMT1X KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MT1X, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMT1X also known as metallothionein-1X is a protein with a molecular mass of approximately 6-7 kDa. This protein belongs to the metallothionein family and plays an essential role in metal ion binding. MT1X primarily expresses in the liver kidneys and pancreas where it helps in stabilizing metal ions and detoxifying heavy metals. The structure of MT1X allows it to bind with metals like zinc and cadmium assisting in maintaining metal homeostasis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMetallothionein-1X acts as a protective agent against oxidative damage by binding and sequestering metal ions therefore preventing the generation of harmful reactive oxygen species. It does not form part of a complex independently but interacts with other proteins like metallothionein-2A to enhance cellular antioxidant defense. MT1X regulates metal ion concentrations within cells and contributes to the detoxification process safeguarding cells against metal-induced cytotoxicity.\u003cbr\u003e\nPathways\u003cbr\u003e\nMT1X functions within pathways related to metal ion metabolism and detoxification processes. In the context of metal ion metabolism it interacts closely with proteins such as zinc transporter proteins maintaining optimal zinc levels in cells. MT1X also associates with the oxidative stress response pathway where it collaborates with proteins like glutathione to mitigate damage from oxidative stress.\u003cbr\u003e\nAlterations in MT1X expression link to disorders like cancer and neurodegenerative diseases. In cancer an altered MT1X expression can impact cellular redox state and metal ion balance contributing to tumor progression. Its potential interplay with proteins like metallothionein-2A may influence cellular responses to oxidative stress in neurodegenerative diseases affecting neuronal survival and function. Understanding MT1X relationships with these proteins might offer insights into the pathogenesis and therapeutic strategies for these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845211115689,"sku":"ab265309","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab265309","provider":"Iright","version":"1.0","type":"link"}