{"product_id":"abcam-ab265320","title":"Abcam, ab265320, Human AGO3 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nAGO3 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 14 bp deletion in exon 12. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 14 bp deletion in exon 12,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-AGO3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAGO3 also known as Argonaute protein 3 is an important component of the RNA-induced silencing complex (RISC). It is part of the Argonaute family which plays a role in RNA silencing processes. AGO3 usually has a molecular mass of around 97 kDa. It is mainly expressed in human tissues such as testes and is responsible for processing small non-coding RNAs including miRNAs and siRNAs. These small RNAs guide AGO3 to complementary RNA sequences facilitating the cleavage and degradation of target mRNA.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAGO3 contributes to gene expression regulation by silencing specific RNA transcripts. This protein forms a complex with other proteins and RNA molecules to execute its functions. Part of the miRNA and RNA interference pathways AGO3 associates with Dicer and other Argonaute proteins creating an effective silencing machinery that modulates various cellular processes. Its activity is essential for maintaining cellular homeostasis through RNA interference-mediated gene silencing.\u003cbr\u003e\nPathways\u003cbr\u003e\nAGO3 plays a significant role in the RNA interference (RNAi) and the microRNA (miRNA) pathways. These pathways are key in post-transcriptional gene regulation controlling gene expression patterns important for development and differentiation. AGO3 interacts with Dicer a pivotal enzyme in the RNAi pathway facilitating the processing of precursor miRNAs into mature miRNAs further guiding RISC to its target mRNA sequences. These interactions highlight AGO3's critical place in RNA silencing networks.\u003cbr\u003e\nAGO3 has links to certain cancers and hereditary disorders. Aberrations in its expression or function can impact miRNA-mediated regulation contributing to tumorigenesis and disorders related to gene expression imbalances. For instance AGO3's dysfunction can relate to testicular cancer due to its significant expression in testicular tissue. Connections between AGO3 and other Argonaute family proteins like AGO1 and AGO2 may influence disease development highlighting its importance in understanding disease mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845108551849,"sku":"ab265320","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab265320","provider":"Iright","version":"1.0","type":"link"}