{"product_id":"abcam-ab265496","title":"Abcam, ab265496, Human OBSCN knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nOBSCN KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-OBSCN, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe OBSCN gene encodes the protein obscurin also known by its alternate names including UNC-89. Obscurin is a large protein with a molecular mass exceeding 800 kDa. It is primarily located in striated muscle where it plays a role in linking the sarcomere to the sarcoplasmic reticulum. Obscurin contains multiple domains that facilitate protein-protein interactions allowing it to interact with various components within muscle cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nObscurin assists in maintaining the structural integrity and organization of myofibrils. It forms part of a complex in muscle cells involving proteins such as titin and myomesin. This complex aids in the mechanical stability of the contractile apparatus ensuring muscle contraction occurs effectively. Obscurin's interactions within this complex contribute to the proper transmission of force across the muscle fiber.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe functions of obscurin link it to the sarcomeric signaling pathway and calcium handling pathways in muscle physiology. These pathways involve coordination with titin and ankyrins which help regulate muscle contraction and relaxation. Through these pathways obscurin influences muscle cell responsiveness to mechanical stress and contributes to muscle homeostasis.\u003cbr\u003e\nAltered obscurin function associates with muscle-related conditions particularly hereditary myopathies and dilated cardiomyopathy. Researchers have linked obscurin to titin due to their shared roles in maintaining sarcomeric structure. Mutations or dysregulations in either protein can lead to insufficient muscle function and structural cardiac issues implicating obscurin as a significant factor in these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845193846953,"sku":"ab265496","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab265496","provider":"Iright","version":"1.0","type":"link"}