{"product_id":"abcam-ab265517","title":"Abcam, ab265517, Human PTGER3 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nPTGER3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 11 bp deletion in exon 1 and 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 11 bp deletion in exon 1 and 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PTGER3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe PTGER3 protein also known as the prostaglandin E2 receptor EP3 subtype is a G-protein coupled receptor (GPCR) with a molecular weight of approximately 53 kDa. This receptor binds prostaglandin E2 (PGE2) and modulates various physiological responses by activating different second messenger systems. PTGER3 exhibits diverse expression patterns being present in tissues such as the smooth muscle kidney uterus neural tissues and certain immune cells. Its expression in various tissues suggests its involvement in multiple functions that rely on PGE2 signaling.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPTGER3 involves itself in mediating the effects of prostaglandin E2 through the inhibition of adenylate cyclase and the modulation of intracellular calcium levels. It does not operate as part of a larger protein complex but functions independently as a receptor. This receptor's activity influences processes such as smooth muscle contraction regulation of body temperature and modulation of hormone release. The variation in signaling allows PTGER3 to have a broad impact on physiological processes adapting its functions based on tissue and cellular context.\u003cbr\u003e\nPathways\u003cbr\u003e\nPTGER3 is part of the prostaglandin signaling pathway and participates in cAMP signaling. It interacts with G-proteins that act as intermediates to modulate adenylate cyclase activity and control cyclic AMP production. The receptor acts in a network with other prostaglandin receptors such as PTGER1 PTGER2 and PTGER4 coordinating responses to PGE2 by either amplifying or opposing their effects depending on the cellular environment. Such interactions position PTGER3 at the junction of pathways critical for homeostatic control and adaptive responses.\u003cbr\u003e\nPTGER3 has connections to several conditions including inflammatory diseases and hypertension. Inflammatory diseases such as rheumatoid arthritis show altered PGE2 signaling pathways where PTGER3 plays a role in mediating inflammation and immune responses. Its activity is also implicated in regulating blood pressure by influencing vascular smooth muscle contraction. Additionally its function intersects with proteins like COX-2 which is involved in PGE2 biosynthesis indicating a broader role in maintaining homeostasis within disease contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845161210025,"sku":"ab265517","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab265517","provider":"Iright","version":"1.0","type":"link"}