{"product_id":"abcam-ab265618","title":"Abcam, ab265618, Human RANBP2 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nRANBP2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 211 bp insertion in exon 1 and 82 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 211 bp insertion in exon 1 and 82 bp insertion in exon 1,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RANBP2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRanBP2 also known as Nucleoporin 358 (Nup358) is a large protein with a molecular mass of approximately 358 kDa. It is an important component of the nuclear pore complex (NPC) and is found on the cytoplasmic side of the nuclear envelope. RanBP2 is expressed in a variety of tissues with higher levels in the brain and testis. Its alternates names include Nup358 due to its role as a nucleoporin. Mechanically RanBP2 facilitates nucleocytoplasmic transport by interacting with the small GTPase Ran.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRanBP2 plays a significant role in multiple processes such as mitotic spindle assembly and chromatin condensation. It functions as part of the Ran GTPase system a critical complex for nucleocytoplasmic transport. In addition RanBP2 contributes to protein sumoylation where it acts as a scaffold for conjugating enzymes. It maintains nuclear envelope integrity and supports the positioning of centrosomes during cell division.\u003cbr\u003e\nPathways\u003cbr\u003e\nRanBP2 is actively involved in the nucleocytoplasmic transport pathway ensuring the proper exchange of proteins and RNA between the nucleus and cytoplasm. It is critical to the Ran cycle where it works alongside proteins like RanGTP and RanGAP1 to regulate transport activities. The involvement of RanBP2 in the SUMOylation pathway also emphasizes its role in post-translational modification impacting various cellular events and signaling.\u003cbr\u003e\nChanges in RanBP2 expression have been linked to neurodegenerative diseases and certain types of cancer. In neurodegenerative conditions such as hereditary spastic paraplegia RanBP2 forms a complex with proteins like atlastin-1 which further influences disease progression. In cancer RanBP2 interacts with oncogenic pathways through its role in cell cycle regulation highlighting its potential as a therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845079060649,"sku":"ab265618","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab265618","provider":"Iright","version":"1.0","type":"link"}