{"product_id":"abcam-ab265657","title":"Abcam, ab265657, Human DPH2 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nDPH2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 20 bp deletion in exon 2 and 7 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 20 bp deletion in exon 2 and 7 bp deletion in exon 2,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-DPH2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDPH2 also known as diphthamide biosynthesis protein 2 functions mechanically in catalyzing the first step of diphthamide biosynthesis. The protein incorporates a unique post-translational modification into histidine residues on elongation factor 2 (EEF2). DPH2 weighs approximately 53 kDa and is expressed in various tissues with high levels seen in the liver and testis. Its structural genes reside on human chromosome 17.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDiphthamide biosynthesis is important to protein synthesis and cellular resilience against bacterial toxins like diphtheria toxin. DPH2 operates within a multiprotein complex including DPH1 and DPH3 necessary for EEF2 modification. Proper function of DPH2 ensures that EEF2 maintains its role in translational fidelity which is indispensable for normal cellular processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nDiphthamide biosynthesis connects to the protein synthesis and modification pathway. DPH2's activities integrate seamlessly with the translational machinery specifically influencing EEF2's function in the ribosome. In addition DPH2 works with DPH1 DPH3 and other proteins in the biosynthesis pathway to fulfill its role efficiently supporting cellular defense mechanisms against translational errors induced by toxins.\u003cbr\u003e\nDefects in DPH2 relate to cancer and neurological conditions. Loss of function mutations in DPH2 can affect cellular processes potentially contributing to tumorigenesis where DPH1 is another connected protein of interest. Genetic alterations in DPH2 can also interfere with neural cell proliferation and survival linking to certain neurodegenerative disorders and emphasizing its role in maintaining cell health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845160390825,"sku":"ab265657","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab265657","provider":"Iright","version":"1.0","type":"link"}