{"product_id":"abcam-ab265839","title":"Abcam, ab265839, Human EXOC2 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nEXOC2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nWestern blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-EXOC2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe exocyst complex component 2 also known as EXOC2 or SEC5 is an important protein involved in vesicle trafficking. EXOC2 has an estimated mass of around 105 kDa. It is widely expressed in various tissues highlighting its broad significance in cellular processes. Its primary role is to participate in the tethering of secretory vesicles to specific sites on the plasma membrane aiding in processes like cell migration and growth.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEXOC2 acts as a fundamental part of the exocyst complex which comprises eight proteins. This complex orchestrates vesicle targeting and tethering during exocytosis a process essential for maintaining cell surface area and membrane protein composition. Unlike other vesicle trafficking complexes the exocyst shows significant specificity for certain vesicles and localization contributing to the precision of membrane-associated functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nEXOC2 joins key pathways like the insulin signaling pathway and the regulation of actin cytoskeleton. In the insulin signaling pathway EXOC2 integrates with components such as the protein Rab11 to mediate glucose transporter vesicle trafficking impacting glucose uptake. In the regulation of the actin cytoskeleton it interacts with actin-related proteins to influence cell shape and motility.\u003cbr\u003e\nConnection with EXOC2 influences conditions such as cancer and diabetes. Abnormal EXOC2 expression links to various cancers often through disruptions in cellular trafficking and signal transduction. The interplay with diabetes arises partly through its role in insulin-mediated processes with proteins like Rab11 that affect glucose homeostasis. These associations highlight the potential for EXOC2 as a therapeutic target in these diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845082370217,"sku":"ab265839","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab265839","provider":"Iright","version":"1.0","type":"link"}