{"product_id":"abcam-ab265933","title":"Abcam, ab265933, Human SIKE1 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nSIKE1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SIKE1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSIKE1 also known as Squamous Cell Carcinoma Antigen Recognized by T-cells 3 (SART3) is a protein with a molecular weight of approximately 24 kDa. It inhibits the kinase activity of Tank-Binding Kinase 1 (TBK1) and IκB kinase ε (IKKε) which are critical for the induction of type I interferons and other cytokines in response to viral infections. SIKE1 is primarily expressed in various tissues including the brain and liver though expression levels may vary depending on cellular context.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSIKE1 interacts with signaling pathways to modulate immune responses. As a negative regulator of the interferon signaling pathway it forms complexes with TBK1\/IKKε to suppress excessive immune activation preventing potential tissue damage. By efficiently regulating kinase activity SIKE1 helps maintain balance in immune signaling ensuring proper immune defense while avoiding overactivation.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of SIKE1 extends to the toll-like receptor (TLR) and retinoic acid-inducible gene I (RIG-I)-like receptor signaling pathways. These pathways play essential roles in detecting and responding to viral pathogens. SIKE1 modulates the activity of TBK1 and IKKε which are central to the aforementioned pathways impacting downstream molecules like IRF3 and NF-kB that drive interferon production and inflammatory responses.\u003cbr\u003e\nSiKE1's regulatory role relates to conditions characterized by dysregulated immune responses. For instance in viral infections where excessive interferon production occurs SIKE1 can mitigate potential tissue damage by dampening cytokine output. It is also studied in auto-inflammatory disorders where control of cytokine production is important for managing disease severity. Its connection to TBK1 and IKKε further implicates SIKE1 in the context of these immune-related conditions as these kinases are targets for therapeutic intervention in disorders related to immune dysregulation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845083123881,"sku":"ab265933","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab265933","provider":"Iright","version":"1.0","type":"link"}