{"product_id":"abcam-ab266033","title":"Abcam, ab266033, Human Plcb1 (Phospholipase C beta 1) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nPLCB1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 5. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 5,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PLCB1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPhospholipase C beta 1 (PLCB1) also known as PLC-β1 or PLCB1 is an enzyme involved in intracellular signal transduction. With a molecular weight of around 150 kDa PLCB1 hydrolyzes phosphatidylinositol 45-bisphosphate (PIP2) into inositol trisphosphate (IP3) and diacylglycerol (DAG). This biochemical activity plays an important role in the release of calcium ions from intracellular stores. PLCB1 is expressed in regions of the brain including the cerebral cortex as well as in other tissues.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMultiple functions depend on the signaling cascades initiated by PLCB1. It belongs to a larger family of phospholipase C enzymes contributing to the regulation of various neural processes. The enzyme acts within multiprotein complexes that modulate intracellular calcium levels influencing neuronal activity and synaptic transmission. This role implicates PLCB1 in modulating brain functions related to learning and memory.\u003cbr\u003e\nPathways\u003cbr\u003e\nPLCB1 contributes to cellular signaling pathways such as the phosphoinositide signaling pathway and the G protein-coupled receptor (GPCR) pathway. In these pathways PLCB1 interacts with major proteins such as Gαq subunit of G proteins which activates it. These interactions place PLCB1 as a central player in the pathways that help convert extracellular signals into intracellular actions often involving the regulation of secondary messengers like IP3 and DAG.\u003cbr\u003e\nResearchers associate PLCB1 with neurological and psychiatric conditions including epilepsy and bipolar disorder. Dysregulation in PLCB1 activity links to altered calcium signaling which can affect brain function. Additionally proteins like Gαq and other GPCR pathway elements may either buffer or exacerbate effects arising from PLCB1 anomalies suggesting molecular interdependencies in related disease mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845107503273,"sku":"ab266033","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266033","provider":"Iright","version":"1.0","type":"link"}