{"product_id":"abcam-ab266129","title":"Abcam, ab266129, Human GRHPR knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nGRHPR KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 29 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 29 bp deletion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GRHPR, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGRHPR also called glyoxylate reductase\/hydroxypyruvate reductase is an enzyme with mass about 35 kDa. It catalyzes the conversion of glyoxylate to glycolate and hydroxypyruvate to D-glycerate. This protein exists in the cytoplasm and is found in tissues like liver kidney and pancreas. Its enzymatic activity plays a role in regulating the balance between glyoxylate and glycolate within cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGRHPR acts as a detoxifying agent by reducing glyoxylate levels protecting cells from glyoxylate-mediated toxicity. This enzyme is not part of a larger complex and functions independently. Well-balanced glyoxylate and glycolate levels are critical for cellular homeostasis and GRHPR ensures proper maintenance and regulation within metabolic pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nGRHPR participates in glyoxylate and dicarboxylate metabolism helping control the flow of metabolites. This involvement is significant in the context of the glyoxylate cycle and serine metabolism. Additionally it interacts with proteins such as lactate dehydrogenase during its activity within these metabolic pathways demonstrating its interconnectedness within cellular processes.\u003cbr\u003e\nGRHPR mutations are associated with primary hyperoxaluria type 2 a disorder characterized by excessive oxalate production leading to kidney stones. In this context GRHPR dysfunction can lead to increased glyoxylate levels causing elevated oxalate synthesis. The enzyme's connection to this disease is critical as it is one of the few proteins whose dysfunction directly results in the metabolic imbalance observed in primary hyperoxaluria type 2.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845193879721,"sku":"ab266129","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266129","provider":"Iright","version":"1.0","type":"link"}