{"product_id":"abcam-ab266134","title":"Abcam, ab266134, Human NANS knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nNANS KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NANS, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nN-acetylneuraminate synthase commonly known as NANS catalyzes the formation of N-acetylneuraminic acid (Neu5Ac) from N-acetyl-D-mannosamine 6-phosphate and phosphoenolpyruvate. This process is important in the sialic acid biosynthesis pathway. NANS has a molecular weight of approximately 46 kDa and is expressed in various tissues most abundantly in the liver and brain. These tissues show significant metabolic activity suggesting the enzyme's active involvement in cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis enzyme plays a significant role in the synthesis of sialic acids which are essential components of glycoconjugates such as glycoproteins and glycolipids. NANS is not known to associate with any specific large protein complexes but its action is necessary for generating substrates that contribute to protein glycosylation and the modification of lipids. These modifications influence cell-cell communication neurite outgrowth and immune response modulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nThis enzyme is pivotal in the sialic acid biosynthesis pathway impacting the production of glycoproteins and glycolipids. In this context NANS interacts with key enzymes like sialyltransferases which further transfer sialic acids to the proteins and lipids. This pathway is essential in glycobiology affecting cellular interactions and signal transduction processes.\u003cbr\u003e\nMutations in the NANS gene have been linked to developmental delay syndromes especially NANS deficiency. These symptoms appear due to disrupted sialic acid production leading to abnormal glycosylation patterns. Furthermore the alteration in NANS activity indirectly affects proteins such as integrins and selectins contributing to immune deficiencies and metabolic syndromes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845197484201,"sku":"ab266134","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266134","provider":"Iright","version":"1.0","type":"link"}