{"product_id":"abcam-ab266142","title":"Abcam, ab266142, Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nHNRNPUL1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon 3 and Insertion of the selection cassette in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon 3 and Insertion of the selection cassette in exon 3\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-HNRNPUL1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe E1B-AP5 protein also known as hnRNPUL1 serves mechanical functions by binding to RNA and DNA and it acts as a regulator in transcription and mRNA processing. This protein weighs approximately 88 kDa. Researchers have identified its presence in the nucleus of different cell types reflecting its central role in nucleic acid metabolism. The protein can also intervene in alternative splicing events and influence mRNA stability making it a significant player within the cell's genetic expression regulation matrix.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nE1B-AP5 integrates into cellular processes as a part of the heterogeneous nuclear ribonucleoproteins complex which facilitates various RNA processing mechanisms. It plays an active role in chromatin structure modulation and RNA transport fostering proper gene expression and maintaining cellular homeostasis. Besides supporting the transcription machinery E1B-AP5 engages in RNA export and surveillance where its presence determines the fate of newly synthesized RNAs.\u003cbr\u003e\nPathways\u003cbr\u003e\nStudies have shown that E1B-AP5 is involved in pathways such as the mRNA surveillance and splicing pathways. It collaborates with proteins like HNRNPA1 and HNRNPC in orchestrating the correct assembly and exportation of RNA transcripts. By participating in these critical pathways E1B-AP5 ensures the fidelity of gene expression and supports the cellular stress response mechanisms contributing to proper cellular function and adaptability.\u003cbr\u003e\nAbnormalities in E1B-AP5 expression correlate with oncogenic transformations and neurodegenerative conditions. In cancer for example dysregulation of E1B-AP5 affects RNA processing and can lead to tumor development with its interaction noted alongside the protein p53 in the context of cellular proliferation control. Understanding E1B-AP5's role in these diseases might provide insights into therapeutic targets particularly where RNA processing and tumor suppressor pathways are implicated.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845066510505,"sku":"ab266142","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266142","provider":"Iright","version":"1.0","type":"link"}