{"product_id":"abcam-ab266227","title":"Abcam, ab266227, Human TMED10 (TMP21) knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nTMED10 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp insertion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TMED10, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTMP21 also known as transmembrane protein 21 or by its alternate name p23 is a protein that plays a significant role in cellular functions. It has an approximate molecular mass of 21 kDa. TMP21 is mainly expressed in the brain but is also found in a variety of other tissues. Mechanically TMP21 functions as a part of the secretory pathway within the cell contributing to the processing and maturation of nascent proteins. It is known to regulate the trafficking of proteins across cellular compartments which is an important aspect of maintaining cellular homeostasis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTMP21 affects the modulation of the gamma-secretase complex which is essential in the generation of amyloid precursor protein (APP) and its subsequent processing. This protein acts as a negative regulator of gamma-secretase activity leading to alterations in APP cleavage. By being part of this complex TMP21 exerts influence over processes such as signal transduction and protein metabolism. Its expression levels and activity can impact neurophysiological functions due to its presence in nervous tissues.\u003cbr\u003e\nPathways\u003cbr\u003e\nTMP21 integrates into the amyloidogenic pathway influencing amyloid-beta peptide production. This pathway is associated with the APP processing where TMP21 regulates the cleavage by controlling gamma-secretase activity. Another pathway involving TMP21 is the protein transport pathway facilitating the orderly movement of proteins through the early secretory pathway. Within these frameworks TMP21 interacts with proteins like presenilin which is a significant component of the gamma-secretase complex thereby affecting its overall functionality.\u003cbr\u003e\nTMP21 has strong links to Alzheimer's disease and cancer. In Alzheimer's disease altered TMP21 function can lead to improper processing of APP resulting in increased amyloid-beta production and plaque formation. This relationship highlights TMP21's connection with presenilin which is important in amyloidogenic processes and influences disease progression. Additionally fluctuations in TMP21 expression and activity have been observed in certain cancers suggesting its role in aberrant cellular growth and migration due to its involvement in protein trafficking pathways. Understanding TMP21's interactions in these diseases can provide insights into potential therapeutic targets.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845067231401,"sku":"ab266227","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266227","provider":"Iright","version":"1.0","type":"link"}