{"product_id":"abcam-ab266242","title":"Abcam, ab266242, Human NUDT5 knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nNUDT5 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 2\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NUDT5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Nudix hydrolase 5 (NUDT5) enzyme also known as ADP-sugar pyrophosphatase performs important mechanical roles by hydrolyzing ADP-ribose to ribose-5-phosphate and AMP. It holds a molecular mass of approximately 39 kDa. NUDT5 shows high expression levels in tissues like liver and ovary. Its activity involves detoxifying nucleoside diphosphate derivatives which prevents their accumulation and potential interference with normal cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNUDT5 influences intracellular signaling pathways and DNA repair processes. It operates as part of multi-enzyme complexes that manage nucleotide metabolism and signaling. By maintaining a balance of ADP-ribose inside cells NUDT5 helps regulate cellular stress responses and genomic stability. Its role in transforming ADP-ribose supports maintaining adequate cellular energy and nucleotide pools for various biological functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nResearch identifies NUDT5 as being involved in the ADP-ribose metabolism pathway and DNA repair mechanisms. In the ADP-ribose pathway NUDT5 assists in the regulation of ADP-ribose levels working alongside other proteins like PARP1 which adds ADP-ribose units to proteins during DNA repair. NUDT5's actions in these pathways ensure the proper functioning of cellular stress responses and the prevention of genome instability.\u003cbr\u003e\nNUDT5 has connections to tumorigenesis and neurodegenerative disorders. Elevated activity of NUDT5 is sometimes observed in certain cancers suggesting a potential role in cancer cell viability. In neurodegenerative diseases such as Parkinson's dysfunctional ADP-ribose metabolism implicates NUDT5. Changes in NUDT5 activity can interact with proteins like PARP1 altering cellular processes that contribute to disease pathogenesis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845079781545,"sku":"ab266242","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266242","provider":"Iright","version":"1.0","type":"link"}