{"product_id":"abcam-ab266256","title":"Abcam, ab266256, Human YDJC knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nYDJC KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-YDJC, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nYDJC also known as Tyrosyl-DNA phosphodiesterase 2 (TDP2) is an enzyme involved in repairing DNA damage by removing covalent protein-DNA adducts. This protein has a mass of approximately 66 kDa. YDJC is expressed in various tissues throughout the body including liver kidney and brain. It plays an essential role in maintaining cellular genomic integrity by enabling the repair of trapped topoisomerase-DNA complexes which can otherwise lead to replication fork stalling or double-strand break formation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nYDJC contributes to cellular DNA repair processes a critical function for cell survival and prevention of mutations. It is not a part of a larger complex functioning independently in its role of cleaving 5'-phosphotyrosyl bonds formed between topoisomerase II and DNA. By facilitating the removal of these protein-DNA lesions YDJC allows the cell to proceed with accurate replication and transcription processes ensuring stability in the genome.\u003cbr\u003e\nPathways\u003cbr\u003e\nYDJC operates within the DNA damage response and repair pathways. Specifically it plays a role in the non-homologous end joining (NHEJ) pathway important for repairing double-strand breaks. YDJC interacts with other proteins such as DNA-PKcs and Ku during this repair process. In addition it is related to the cell cycle regulation pathway where its function is to prevent the accumulation of DNA damage therefore aiding in maintaining cell cycle integrity.\u003cbr\u003e\nYDJC has connections to cancer and neurodegenerative diseases. In cancer the efficient repair of DNA damage by YDJC can influence tumor resistance to certain chemotherapeutic agents. Aberrant expression of YDJC can lead to increased DNA damage resistance in tumor cells potentially leading to chemotherapy resistance. Furthermore in neurodegenerative diseases the failure to repair DNA damage due to impaired YDJC function may contribute to neuronal cell death. YDJC's activity links it to the XRCC1 protein which is involved in maintaining neuronal health through DNA repair mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845197123753,"sku":"ab266256","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266256","provider":"Iright","version":"1.0","type":"link"}