{"product_id":"abcam-ab266422","title":"Abcam, ab266422, Human TPM3 (Tropomyosin 3) knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nTPM3 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TPM3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTropomyosin 3 also known as TPM3 is an actin-binding protein that stabilizes actin filaments. This protein plays an important role in maintaining cytoskeleton integrity and regulating cell contraction. Tropomyosin 3 has a molecular weight of approximately 32.5 kDa and is widely expressed in various tissues especially in muscle cells and in some non-muscle tissues. This protein interacts with other proteins to form complexes that modulate the dynamics of the actin filament systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTropomyosin 3 modulates the access of other actin-binding proteins to the actin filament by covering and uncovering binding sites. It partners with actin and troponin complexes influencing muscle contraction and cell motility. Additionally TPM3 regulates the stability and flexibility of actin cortex important for cell shape and movement. In non-muscle cells it supports the formation of cell structures needed for signaling and transportation.\u003cbr\u003e\nPathways\u003cbr\u003e\nTropomyosin 3 functions in the regulation of the actin cytoskeleton pathway and the muscle contraction pathway. It coordinates with proteins like actin and myosin to facilitate these processes. In the actin cytoskeleton pathway TPM3 balances polymerization and depolymerization of actin important for maintaining cellular structures. The muscle contraction pathway relies on TPM3 to regulate calcium binding supporting muscular function and responsiveness.\u003cbr\u003e\nTropomyosin 3 mutations link to conditions like congenital myopathy and nemaline myopathy. Mutations in TPM3 affect muscle fiber regulation leading to muscle weakness and structural abnormalities. Congenital myopathy involves other proteins such as nebulin which cooperate with TPM3 in maintaining muscle architecture. In nemaline myopathy improper expression or structure of TPM3 contributes to defective sarcomere formation impairing muscle function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845108781225,"sku":"ab266422","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266422","provider":"Iright","version":"1.0","type":"link"}