{"product_id":"abcam-ab266549","title":"Abcam, ab266549, Human BRPF1 (Peregrin) knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nBRPF1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 34 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 34 bp deletion in exon 2\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-BRPF1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPeregrin\/BRPF1 also known as Peregrins is a protein with a molecular mass around 130 kDa. It functions primarily as a component of the MOZ\/MORF histone acetyltransferase (HAT) complex. Peregrin\/BRPF1 plays a mechanical role in the regulation of gene expression by altering chromatin structure through histone modification. It is expressed in various tissues with higher expression in the brain and testis indicating its importance in neurological and reproductive functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPeregrin\/BRPF1 is a part of the MOZ\/MORF complex which is important for acetylating histone H3 at lysine 23 influencing transcriptional activation. This action aids in transcription regulation and chromatin remodeling facilitating access of transcription factors to DNA. By participating in these processes Peregrin\/BRPF1 helps maintain proper gene expression patterns essential for normal cell function and development.\u003cbr\u003e\nPathways\u003cbr\u003e\nBRPF1 has roles in processes like the cell cycle and embryonic development pathways because its histone acetylation influences gene expression. It interacts with other acetyltransferases such as EP300 and CREBBP which play key roles in transcription regulation and chromatin remodeling within these pathways. These interactions highlight BRPF1's critical involvement in coordinating cellular events and developmental processes.\u003cbr\u003e\nDysregulation of BRPF1 is associated with neurological disorders such as intellectual disability and specific cancers due to its role in chromatin remodeling and gene expression. In the context of intellectual disability it interacts with other proteins involved in chromatin structure like NSD1 and KDM6A. These connections illustrate the significant impact of Peregrin\/BRPF1's regulation and its influence on health when its function becomes impaired.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845057564841,"sku":"ab266549","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266549","provider":"Iright","version":"1.0","type":"link"}