{"product_id":"abcam-ab266572","title":"Abcam, ab266572, Human TMEM165 knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nTMEM165 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp insertion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TMEM165, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTMEM165 also known as Transmembrane Protein 165 or TMPTD is a transmembrane protein with an approximate molecular mass of 41 kDa. This protein predominantly localizes to the Golgi apparatus where it plays a mechanical function as a cation transporter. Expression of TMEM165 is wide with the protein being found in various tissues but with higher representation in certain organs including the heart and liver. Its structure comprises multiple transmembrane domains contributing to its role in maintaining ion homeostasis within the organelles.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTMEM165 maintains the balance of ions within the Golgi apparatus influencing glycosylation processes. It is part of a complex that includes ion channels and other transporters essential for the proper modification of proteins through glycosylation. Alterations in TMEM165 function could affect the fidelity of these protein modifications impacting cell signaling and function. These roles emphasize TMEM165's importance in cellular activities that rely on precisely glycosylated proteins.\u003cbr\u003e\nPathways\u003cbr\u003e\nTMEM165 plays significant roles in both glycosylation and electrolyte balance within the Golgi apparatus. It participates in pathways related to calcium and manganese ion homeostasis important for Golgi functions and protein processing. The protein interacts with other ion transporters and channels such as ATP2C1 to facilitate these processes. These interactions support proper folding and processing of glycoproteins which is integral to maintaining cellular and tissue homeostasis.\u003cbr\u003e\nThe malfunction of TMEM165 links to complex syndromes such as Congenital Disorder of Glycosylation (CDG) where improper protein glycosylation occurs. Mutations in the TMEM165 gene result in defective glycosylation leading to various clinical manifestations. Another disorder related to TMEM165 is characterized by bone development issues possibly connected to its role in manganese transport. Researchers have also observed interactions with TMEM51 regarding skeletal abnormalities indicating a shared pathway alteration contributing to disease development.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845206462633,"sku":"ab266572","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266572","provider":"Iright","version":"1.0","type":"link"}