{"product_id":"abcam-ab266606","title":"Abcam, ab266606, Human TPM4 knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nTPM4 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TPM4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTPM4 also known as tropomyosin 4 or TM4 is a protein that helps regulate muscle contraction and stabilize actin filaments. It has a molecular mass of approximately 28 kDa. TPM4 expression occurs in various tissues including both skeletal and smooth muscle tissues as well as in non-muscle cells. This protein is part of a larger family of tropomyosins which bind to actin filaments and play an important role in modulating actin's interaction with other proteins.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTPM4 contributes to the regulation of the actin cytoskeleton dynamics influencing cell movement and shape. TPM4 like other tropomyosins associates with actin filaments and forms a part of a complex with other actin-binding proteins such as tropomodulins. This complex is critical in both maintaining filament stability and facilitating the sliding of actin filaments during muscle contraction and other cellular activities.\u003cbr\u003e\nPathways\u003cbr\u003e\nTPM4 participates actively in the cytoskeletal signaling and muscle contraction pathways. It interacts with important components in these pathways such as myosin allowing coordinated muscle contractions. TPM4 also plays a role in the regulation of the RhoA signaling pathway which is connected to cytoskeletal rearrangement and cell motility. These interactions highlight TPM4's integral position in biological processes relying on actin filament dynamics.\u003cbr\u003e\nMisregulation of TPM4 can influence the development of conditions such as cardiomyopathies and cancer. Alterations in its expression or function may link to familial hypertrophic cardiomyopathy where it interacts with cardiac-specific proteins like cardiac myosin heavy chain (MYH7). Furthermore changes in TPM4 expression levels may associate with cancer metastasis in part due to its role in cell motility and invasion pointing to potential connections with proteins like cofilin during cancer progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845066969257,"sku":"ab266606","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266606","provider":"Iright","version":"1.0","type":"link"}