{"product_id":"abcam-ab266614","title":"Abcam, ab266614, Human ARF5 knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nARF5 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 13 bp deletion in exon 1 and 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 13 bp deletion in exon 1 and 1 bp insertion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ARF5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe ADP-ribosylation factor 5 (ARF5) protein also known by its primary name is an important part of the ARF family. It has a mass of around 20 kDa. ARF5 plays a role in regulating vesicle trafficking systems particularly linked with the Golgi apparatus. It is found broadly expressed in numerous tissues with high levels observed in the brain and testes. This small GTP-binding protein is associated with intracellular signaling cascades and cellular communication.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nARF5 is involved in modulating key cellular processes including membrane trafficking and lipid metabolism. It directly participates in the formation of vesicle coats by aiding the recruitment of coat protein complexes. ARF5 also interacts within cellular complexes impacting the transport of cargo in the secretory pathway. It also affects actin cytoskeleton remodeling thereby influencing cell shape and motility.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe ARF5 protein contributes to both the COPI and clathrin-coated vesicle formation pathways. It functions within intricate signaling networks coordinating with other small GTPases such as ARF1. ARF5's activity links to pivotal roles within these pathways affecting protein and lipid sorting. These pathways are vital for maintaining cellular homeostasis and facilitating effective intracellular transport.\u003cbr\u003e\nARF5's dysfunction has been linked to neurodegenerative disorders including Alzheimer's disease. Variations in the expression or mutations of ARF5 might contribute to the disease pathology by impairing vesicular transport and potentially interacting with proteins like tau. Furthermore ARF5 may relate to metabolic syndromes due to its involvement in lipid metabolism with implications for body fat distribution and lipid-related diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845122707625,"sku":"ab266614","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266614","provider":"Iright","version":"1.0","type":"link"}