{"product_id":"abcam-ab266855","title":"Abcam, ab266855, Human ZFYVE16 (Endofin) knockout HCT116 cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nZFYVE16 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HCT116,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Colon,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 3,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ZFYVE16, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEndofin also known as ZFYVE16 plays a role in cellular signaling processes. This protein contains a FYVE domain which allows it to bind phosphatidylinositol 3-phosphate a lipid that is important for cellular trafficking. Endofin has a mass of approximately 85 kDa. Researchers have found Endofin expressed in various tissues including the brain heart and kidney indicating its widespread functional importance in different biological systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEndosomes rely on Endofin for maturation and protein sorting. Endofin acts as a scaffold protein within endosomal complexes helping direct the trafficking of proteins within the cell. It interacts with SMAD proteins which play role in TGF-beta signaling and helps mediate signal transduction from the cell surface to the nucleus. Through its interactions Endofin influences important cellular processes like cell growth differentiation and apoptosis.\u003cbr\u003e\nPathways\u003cbr\u003e\nEndosomal dynamics integrate Endofin into the TGF-beta signaling pathway by its association with SMAD proteins. Endofin modulates the pathway by facilitating the phosphorylation and nuclear translocation of these SMAD proteins. Endofin also relates to the BMP signaling pathway and affects the regulation of cellular responses to developmental signals. Through its pathway integration Endofin regulates both physiological processes and responses to extracellular signals.\u003cbr\u003e\nDysfunctions in Endofin can lead to cancer and neurodegenerative diseases. Its involvement in the TGF-beta signaling pathway connects Endofin to tumor progression since defects in this pathway often result in uncontrolled cell proliferation. Furthermore Endofin interacts with Huntington's disease protein hinting at a potential link to molecular mechanisms underlying neurodegenerative diseases. Understanding Endofin and its pathway connections may aid in developing therapeutic interventions for these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845196927145,"sku":"ab266855","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266855","provider":"Iright","version":"1.0","type":"link"}