{"product_id":"abcam-ab266911","title":"Abcam, ab266911, Human RNF19B knockout A549 cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nRNF19B KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 2 bp deletion in exon 2,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RNF19B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRNF19B also known as RING finger protein 19B or Dorfin is an E3 ubiquitin-protein ligase that functions through tagging various substrates with ubiquitin marking them for degradation by the proteasome. It has a molecular weight of approximately 54 kDa. This protein appears in various tissues but shows higher expression in the brain spinal cord heart and muscle. RNF19B localizes to the cytoplasm and is known for its role in regulating several cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRNF19B participates in the endoplasmic reticulum-associated degradation (ERAD) pathway and interacts with different co-factors to form functional complexes. It facilitates the removal of misfolded or damaged proteins within the cell thereby maintaining protein homeostasis. The protein's ability to recognize and ubiquitinate misfolded proteins plays a critical part in cellular quality control mechanisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nRNF19B interacts significantly with the ubiquitin-proteasome system and also affects the Parkinson's disease pathway. RNF19B links to key proteins like parkin and CHIP which are involved in the degradation of proteins associated with neurodegenerative diseases. Through these interactions RNF19B modulates cell health and protein turnover highlighting its central role in maintaining cellular balance.\u003cbr\u003e\nRNF19B exhibits a strong connection to neurodegenerative conditions like amyotrophic lateral sclerosis (ALS) and Parkinson's disease. Mutations or dysregulation of RNF19B can exacerbate cellular stress contributing to disease progression. Additionally RNF19B associates with the protein huntingtin impacting pathways related to Huntington's disease. The protein's ability to regulate misfolded protein degradation links it directly to these disease states suggesting its potential as a therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845196271785,"sku":"ab266911","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/abcam-ab266911","provider":"Iright","version":"1.0","type":"link"}